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cho cd efficient feed a  (Thermo Fisher)


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    Thermo Fisher cho cd efficient feed a
    Cho Cd Efficient Feed A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cho+cd+efficient+feed+a/cho+cd+efficient+feed+a/pm40403225-58-13-20
    Average 90 stars, based on 1 article reviews
    cho cd efficient feed a - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Anti-BTN3A antibodies and their use in treating cancer or infectious disorders
    Article Snippet: Cultures were fed daily by the addition of 3.6% (of the starting volume) feed (2.5% CHO CD Efficient Feed A (ThermoFisher, Loughborough, UK), 0.5% Yeastolate (BD Biosciences, Oxford, UK), 0.25 mM Glutamax (ThermoFisher, Loughborough, UK) and 2 g/L Glucose (Sigma, Dorset, UK)).

    Article Title: BTN3A binding proteins and uses thereof
    Article Snippet: Cultures were fed daily by the addition of 3.6% (of the starting volume) feed (2.5% CHO CD Efficient Feed A (ThermoFisher, Loughborough, UK), 0.5% Yeastolate (BD Biosciences, Oxford, UK), 0.25 mM Glutamax (ThermoFisher, Loughborough, UK) and 2 g/L Glucose (Sigma, Dorset, UK).

    Article Title: Anti-BTN3A antibodies and their use in treating cancer or infectious disorders
    Article Snippet: Cultures were fed daily by the addition of 3.6% (of the starting volume) feed (2.5% CHO CD Efficient Feed A (ThermoFisher, Loughborough, UK), 0.5% Yeastolate (BD Biosciences, Oxford, UK), 0.25 mM Glutamax (ThermoFisher, Loughborough, UK) and 2 g/L Glucose (Sigma, Dorset, UK)).

    Article Title: Development of a Novel Engineered Antibody Format for PSMA-Targeted Radionuclide Therapy.
    Article Snippet: Prostate cancer remains a prevalent and lethal malignancy across the globe.. Despite ongoing advances in therapeutic approaches, these remain ineffective, and new treatments are drastically needed.. Prostate-specific membrane antigen (PSMA)-targeted radionuclide therapy is a well-developed approach for prostate cancer treatment; however, current small molecule and antibody carriers for molecular radiotherapy each have drawbacks in their biodistribution and consequent side effects as highlighted in current clinical trials.

    Article Title: Impact of hydrolysates on monoclonal antibody productivity, purification and quality in Chinese hamster ovary cells.
    Article Snippet: Please cite Chinese ha Plant and yeast derived hydrolysates are economical and efficient alternative medium supplements to improve mammalian cell culture performance.. We supplemented two commercial Chinese hamster ovary (CHO) culture media with hydrolysates from four different sources, yeast, soybean, Ex-Cell CD (a chemically defined hydrolysate replacement) and wheat to improve the productivity of two cell lines expressing different monoclonal antibodies (mAbs).. Yeast, soybean and Ex-Cell CD improved the final mAb titer by increasing the specific productivity (qP) and/or extension of the culture period.

    Article Title: GITRL fusion proteins comprising a human coronin 1a derived trimerization domain
    Article Snippet: The cultures were maintained as a fed batch, using a nutrient feed similar to CHO CD-Efficient Feed ATM (Life Technologies Ltd, Paisley, UK), for 10-12 days at which point they were harvested using filtration for cell removal.

    Polymerase Chain Reaction:

    Article Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations
    Article Snippet: Reagent or Resource Source Identifier Antibodies goat-anti-hIgG(Fc)-HRP Sigma Cat#A0170; RRID:AB_257868 murine mAb 9E10 Expressed for this paper in-house, original (Chan et al., 1987) N/A goat-anti-mIgG(Fc)-HRP Sigma Cat#A0168; RRID:AB_257867 Hyper-Myc (TUN219-2C1-mFc) Abcalis Cat#ABC0503 Avelumab Expressed for this paper in-house, original (Boyerinas et al., 2015) N/A Palivizumab Expressed for this paper in-house, original (Subramanian et al., 1998) N/A STE90-C11 This paper N/A mouse anti-His epitope tag Dianova Cat#DIA-900-200 mounse anti-penta His QIAGEN Cat#34660; RRID:AB_2619735 goat anti-hIgG(Fc)-AP Jackson ImmunoResearch Cat#AB_2337599; RRID:AB_2337599 goat anti-mIgG-AP Dianova Cat#115-055-071; RRID:AB_2338535 mouse a-CD138 (FITC conjugated) BioLegend Cat#352304; RRID:AB_10900441 Bacterial and virus strains E. coli XL1 Blue MRF’ Stratagene Cat#200230 E. coli TG1 Lucigen Cat#605021 SARS-CoV-2/M€unster/FI110320/1/2020 University of M€unster, Germany N/A SARS-CoV-2 BetaCoV/Germany/ BavPat1/2020 Charité, Germany N/A Hyperphage Produced in-house N/A M13KO7 helperphage Produced in-house N/A Experimental model: cell lines Expi293F Thermo Fisher Scientific Cat#A14527 VeroE6 ATCC ATCC CRL-1586 High Five Thermo Fisher Scientific Cat#B85502 Biological samples COVID-19 patient samples local hospitals N/A COVID-19 immune libraries This paper N/A Healthy adult control samples (from preCOVID-19 time) LADR GmbH Medizinisches Versorgungszentrum Braunschweig N/A Chemicals, peptides, and recombinant proteins Spike protein (RBD; S1; S1-S2; N) This paper GenBank: MN908947 ACE2 This paper GenBank: NM021804.3 PEI 40 kDa Polysciences Cat#24765-1 Ficoll GE Healthcare Cat#45-001-749 TRIzol LS Life Technologies Cat#10296028 Superscript IV Thermo Fisher Scientific Cat#18090010 Trypsin Sigma Cat#9002-07-7 IPTG (50 mM) Sigma Cat#367-93-1 GIBCO FreeStyle F17 expression media Thermo Fisher Scientific Cat#10388033 HyClone SFM4Transfx-293 media GE Healthcare Cat#10015813 HyClone Boost 6 supplement GE Healthcare Cat#11576341 (Continued on next page) Cell Reports 36, 109433, July 27, 2021 e1 .. Reagent or Resource Source Identifier Laemmli sample buffer Laemmli, 1970 N/A 5-Brom-4-Chlor-3-Indolyl-Phosphat/Nitro Tetrazolium Blue Chloride Thermo Fisher Scientific Cat#B6149 CHO CD efficient feed A Thermo Fisher Scientific Cat#A1023401 ExCell 405 serum-free medium Sigma Cat#14405C 2,3-(R,R)-butandiol Alfa Aeser Cat#513-85-9 Critical commercial kits Direct-zol RNA Miniprep Plus Kit Zymo Research Cat#R2072 NucleoBond Xtra Midi Kit Macherey-Nagel Cat#12798402 NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#11992242 NucleoSpin Plasmid EasyPure Macherey-Nagel Cat#15162537 NEB Luna Universal Probe One-Step RT-qPCR kit NEB Cat#E3006S Deposited data Crystal structure of STE90-C11 in complex with SARS-CoV-2-RBD This paper PDB 7B3O Oligonucleotides Primer set for VH and VL amplification (Wenzel et al., 2020b) N/A Recombinant DNA Spike protein (RBD; S1; S1-S2; N) Thermo Fisher/Gene Art GenBank: MN908947 ACE2 Thermo Fisher/Gene Art GenBank: NM021804.3 Software and algorithms OrginPro (2019) OrginLab N/A GraphPad Prism 6.1 GraphPad https://www.graphpad.com VBASE MRC Centre for Protein Engineering https://www2.mrc-lmb.cam.ac.uk/vbase/ VBASE2 (Mollova et al., 2010) www.vbase2.org DataGraph (4.5.1) DataGraph https://www.visualdatatools.com/ DataGraph/ IncuCyte S3 GUI Sartorius N/A Phenix (Liebschner et al., 2019) www.phenix-online.org BD FACS Chorus BD N/A PyMol Schrödinger LLC www.pymol.org/2/ PISA European Bioinformatics Institute https://www.ebi.ac.uk/pdbe/ prot_int/pistart.html Octet qKe Data Analysis HT 11.0 Fortebio/Sartorius N/A Other Äkta Go/Äkta Pure Cytiva N/A Profinia System BIO-RAD N/A Precision XS microplate sample processor BioTek N/A Sartorius IncuCyte S3 Sartorius N/A BD FACSMelody Cell Sorter BD N/A Octet qKe Fortebio/Sartorius N/A PETRA III DESY Hamburg N/A Article ll OPEN ACCESS ..

    Plasmid Preparation:

    Article Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations
    Article Snippet: Reagent or Resource Source Identifier Antibodies goat-anti-hIgG(Fc)-HRP Sigma Cat#A0170; RRID:AB_257868 murine mAb 9E10 Expressed for this paper in-house, original (Chan et al., 1987) N/A goat-anti-mIgG(Fc)-HRP Sigma Cat#A0168; RRID:AB_257867 Hyper-Myc (TUN219-2C1-mFc) Abcalis Cat#ABC0503 Avelumab Expressed for this paper in-house, original (Boyerinas et al., 2015) N/A Palivizumab Expressed for this paper in-house, original (Subramanian et al., 1998) N/A STE90-C11 This paper N/A mouse anti-His epitope tag Dianova Cat#DIA-900-200 mounse anti-penta His QIAGEN Cat#34660; RRID:AB_2619735 goat anti-hIgG(Fc)-AP Jackson ImmunoResearch Cat#AB_2337599; RRID:AB_2337599 goat anti-mIgG-AP Dianova Cat#115-055-071; RRID:AB_2338535 mouse a-CD138 (FITC conjugated) BioLegend Cat#352304; RRID:AB_10900441 Bacterial and virus strains E. coli XL1 Blue MRF’ Stratagene Cat#200230 E. coli TG1 Lucigen Cat#605021 SARS-CoV-2/M€unster/FI110320/1/2020 University of M€unster, Germany N/A SARS-CoV-2 BetaCoV/Germany/ BavPat1/2020 Charité, Germany N/A Hyperphage Produced in-house N/A M13KO7 helperphage Produced in-house N/A Experimental model: cell lines Expi293F Thermo Fisher Scientific Cat#A14527 VeroE6 ATCC ATCC CRL-1586 High Five Thermo Fisher Scientific Cat#B85502 Biological samples COVID-19 patient samples local hospitals N/A COVID-19 immune libraries This paper N/A Healthy adult control samples (from preCOVID-19 time) LADR GmbH Medizinisches Versorgungszentrum Braunschweig N/A Chemicals, peptides, and recombinant proteins Spike protein (RBD; S1; S1-S2; N) This paper GenBank: MN908947 ACE2 This paper GenBank: NM021804.3 PEI 40 kDa Polysciences Cat#24765-1 Ficoll GE Healthcare Cat#45-001-749 TRIzol LS Life Technologies Cat#10296028 Superscript IV Thermo Fisher Scientific Cat#18090010 Trypsin Sigma Cat#9002-07-7 IPTG (50 mM) Sigma Cat#367-93-1 GIBCO FreeStyle F17 expression media Thermo Fisher Scientific Cat#10388033 HyClone SFM4Transfx-293 media GE Healthcare Cat#10015813 HyClone Boost 6 supplement GE Healthcare Cat#11576341 (Continued on next page) Cell Reports 36, 109433, July 27, 2021 e1 .. Reagent or Resource Source Identifier Laemmli sample buffer Laemmli, 1970 N/A 5-Brom-4-Chlor-3-Indolyl-Phosphat/Nitro Tetrazolium Blue Chloride Thermo Fisher Scientific Cat#B6149 CHO CD efficient feed A Thermo Fisher Scientific Cat#A1023401 ExCell 405 serum-free medium Sigma Cat#14405C 2,3-(R,R)-butandiol Alfa Aeser Cat#513-85-9 Critical commercial kits Direct-zol RNA Miniprep Plus Kit Zymo Research Cat#R2072 NucleoBond Xtra Midi Kit Macherey-Nagel Cat#12798402 NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#11992242 NucleoSpin Plasmid EasyPure Macherey-Nagel Cat#15162537 NEB Luna Universal Probe One-Step RT-qPCR kit NEB Cat#E3006S Deposited data Crystal structure of STE90-C11 in complex with SARS-CoV-2-RBD This paper PDB 7B3O Oligonucleotides Primer set for VH and VL amplification (Wenzel et al., 2020b) N/A Recombinant DNA Spike protein (RBD; S1; S1-S2; N) Thermo Fisher/Gene Art GenBank: MN908947 ACE2 Thermo Fisher/Gene Art GenBank: NM021804.3 Software and algorithms OrginPro (2019) OrginLab N/A GraphPad Prism 6.1 GraphPad https://www.graphpad.com VBASE MRC Centre for Protein Engineering https://www2.mrc-lmb.cam.ac.uk/vbase/ VBASE2 (Mollova et al., 2010) www.vbase2.org DataGraph (4.5.1) DataGraph https://www.visualdatatools.com/ DataGraph/ IncuCyte S3 GUI Sartorius N/A Phenix (Liebschner et al., 2019) www.phenix-online.org BD FACS Chorus BD N/A PyMol Schrödinger LLC www.pymol.org/2/ PISA European Bioinformatics Institute https://www.ebi.ac.uk/pdbe/ prot_int/pistart.html Octet qKe Data Analysis HT 11.0 Fortebio/Sartorius N/A Other Äkta Go/Äkta Pure Cytiva N/A Profinia System BIO-RAD N/A Precision XS microplate sample processor BioTek N/A Sartorius IncuCyte S3 Sartorius N/A BD FACSMelody Cell Sorter BD N/A Octet qKe Fortebio/Sartorius N/A PETRA III DESY Hamburg N/A Article ll OPEN ACCESS ..

    Quantitative RT-PCR:

    Article Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations
    Article Snippet: Reagent or Resource Source Identifier Antibodies goat-anti-hIgG(Fc)-HRP Sigma Cat#A0170; RRID:AB_257868 murine mAb 9E10 Expressed for this paper in-house, original (Chan et al., 1987) N/A goat-anti-mIgG(Fc)-HRP Sigma Cat#A0168; RRID:AB_257867 Hyper-Myc (TUN219-2C1-mFc) Abcalis Cat#ABC0503 Avelumab Expressed for this paper in-house, original (Boyerinas et al., 2015) N/A Palivizumab Expressed for this paper in-house, original (Subramanian et al., 1998) N/A STE90-C11 This paper N/A mouse anti-His epitope tag Dianova Cat#DIA-900-200 mounse anti-penta His QIAGEN Cat#34660; RRID:AB_2619735 goat anti-hIgG(Fc)-AP Jackson ImmunoResearch Cat#AB_2337599; RRID:AB_2337599 goat anti-mIgG-AP Dianova Cat#115-055-071; RRID:AB_2338535 mouse a-CD138 (FITC conjugated) BioLegend Cat#352304; RRID:AB_10900441 Bacterial and virus strains E. coli XL1 Blue MRF’ Stratagene Cat#200230 E. coli TG1 Lucigen Cat#605021 SARS-CoV-2/M€unster/FI110320/1/2020 University of M€unster, Germany N/A SARS-CoV-2 BetaCoV/Germany/ BavPat1/2020 Charité, Germany N/A Hyperphage Produced in-house N/A M13KO7 helperphage Produced in-house N/A Experimental model: cell lines Expi293F Thermo Fisher Scientific Cat#A14527 VeroE6 ATCC ATCC CRL-1586 High Five Thermo Fisher Scientific Cat#B85502 Biological samples COVID-19 patient samples local hospitals N/A COVID-19 immune libraries This paper N/A Healthy adult control samples (from preCOVID-19 time) LADR GmbH Medizinisches Versorgungszentrum Braunschweig N/A Chemicals, peptides, and recombinant proteins Spike protein (RBD; S1; S1-S2; N) This paper GenBank: MN908947 ACE2 This paper GenBank: NM021804.3 PEI 40 kDa Polysciences Cat#24765-1 Ficoll GE Healthcare Cat#45-001-749 TRIzol LS Life Technologies Cat#10296028 Superscript IV Thermo Fisher Scientific Cat#18090010 Trypsin Sigma Cat#9002-07-7 IPTG (50 mM) Sigma Cat#367-93-1 GIBCO FreeStyle F17 expression media Thermo Fisher Scientific Cat#10388033 HyClone SFM4Transfx-293 media GE Healthcare Cat#10015813 HyClone Boost 6 supplement GE Healthcare Cat#11576341 (Continued on next page) Cell Reports 36, 109433, July 27, 2021 e1 .. Reagent or Resource Source Identifier Laemmli sample buffer Laemmli, 1970 N/A 5-Brom-4-Chlor-3-Indolyl-Phosphat/Nitro Tetrazolium Blue Chloride Thermo Fisher Scientific Cat#B6149 CHO CD efficient feed A Thermo Fisher Scientific Cat#A1023401 ExCell 405 serum-free medium Sigma Cat#14405C 2,3-(R,R)-butandiol Alfa Aeser Cat#513-85-9 Critical commercial kits Direct-zol RNA Miniprep Plus Kit Zymo Research Cat#R2072 NucleoBond Xtra Midi Kit Macherey-Nagel Cat#12798402 NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#11992242 NucleoSpin Plasmid EasyPure Macherey-Nagel Cat#15162537 NEB Luna Universal Probe One-Step RT-qPCR kit NEB Cat#E3006S Deposited data Crystal structure of STE90-C11 in complex with SARS-CoV-2-RBD This paper PDB 7B3O Oligonucleotides Primer set for VH and VL amplification (Wenzel et al., 2020b) N/A Recombinant DNA Spike protein (RBD; S1; S1-S2; N) Thermo Fisher/Gene Art GenBank: MN908947 ACE2 Thermo Fisher/Gene Art GenBank: NM021804.3 Software and algorithms OrginPro (2019) OrginLab N/A GraphPad Prism 6.1 GraphPad https://www.graphpad.com VBASE MRC Centre for Protein Engineering https://www2.mrc-lmb.cam.ac.uk/vbase/ VBASE2 (Mollova et al., 2010) www.vbase2.org DataGraph (4.5.1) DataGraph https://www.visualdatatools.com/ DataGraph/ IncuCyte S3 GUI Sartorius N/A Phenix (Liebschner et al., 2019) www.phenix-online.org BD FACS Chorus BD N/A PyMol Schrödinger LLC www.pymol.org/2/ PISA European Bioinformatics Institute https://www.ebi.ac.uk/pdbe/ prot_int/pistart.html Octet qKe Data Analysis HT 11.0 Fortebio/Sartorius N/A Other Äkta Go/Äkta Pure Cytiva N/A Profinia System BIO-RAD N/A Precision XS microplate sample processor BioTek N/A Sartorius IncuCyte S3 Sartorius N/A BD FACSMelody Cell Sorter BD N/A Octet qKe Fortebio/Sartorius N/A PETRA III DESY Hamburg N/A Article ll OPEN ACCESS ..

    Amplification:

    Article Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations
    Article Snippet: Reagent or Resource Source Identifier Antibodies goat-anti-hIgG(Fc)-HRP Sigma Cat#A0170; RRID:AB_257868 murine mAb 9E10 Expressed for this paper in-house, original (Chan et al., 1987) N/A goat-anti-mIgG(Fc)-HRP Sigma Cat#A0168; RRID:AB_257867 Hyper-Myc (TUN219-2C1-mFc) Abcalis Cat#ABC0503 Avelumab Expressed for this paper in-house, original (Boyerinas et al., 2015) N/A Palivizumab Expressed for this paper in-house, original (Subramanian et al., 1998) N/A STE90-C11 This paper N/A mouse anti-His epitope tag Dianova Cat#DIA-900-200 mounse anti-penta His QIAGEN Cat#34660; RRID:AB_2619735 goat anti-hIgG(Fc)-AP Jackson ImmunoResearch Cat#AB_2337599; RRID:AB_2337599 goat anti-mIgG-AP Dianova Cat#115-055-071; RRID:AB_2338535 mouse a-CD138 (FITC conjugated) BioLegend Cat#352304; RRID:AB_10900441 Bacterial and virus strains E. coli XL1 Blue MRF’ Stratagene Cat#200230 E. coli TG1 Lucigen Cat#605021 SARS-CoV-2/M€unster/FI110320/1/2020 University of M€unster, Germany N/A SARS-CoV-2 BetaCoV/Germany/ BavPat1/2020 Charité, Germany N/A Hyperphage Produced in-house N/A M13KO7 helperphage Produced in-house N/A Experimental model: cell lines Expi293F Thermo Fisher Scientific Cat#A14527 VeroE6 ATCC ATCC CRL-1586 High Five Thermo Fisher Scientific Cat#B85502 Biological samples COVID-19 patient samples local hospitals N/A COVID-19 immune libraries This paper N/A Healthy adult control samples (from preCOVID-19 time) LADR GmbH Medizinisches Versorgungszentrum Braunschweig N/A Chemicals, peptides, and recombinant proteins Spike protein (RBD; S1; S1-S2; N) This paper GenBank: MN908947 ACE2 This paper GenBank: NM021804.3 PEI 40 kDa Polysciences Cat#24765-1 Ficoll GE Healthcare Cat#45-001-749 TRIzol LS Life Technologies Cat#10296028 Superscript IV Thermo Fisher Scientific Cat#18090010 Trypsin Sigma Cat#9002-07-7 IPTG (50 mM) Sigma Cat#367-93-1 GIBCO FreeStyle F17 expression media Thermo Fisher Scientific Cat#10388033 HyClone SFM4Transfx-293 media GE Healthcare Cat#10015813 HyClone Boost 6 supplement GE Healthcare Cat#11576341 (Continued on next page) Cell Reports 36, 109433, July 27, 2021 e1 .. Reagent or Resource Source Identifier Laemmli sample buffer Laemmli, 1970 N/A 5-Brom-4-Chlor-3-Indolyl-Phosphat/Nitro Tetrazolium Blue Chloride Thermo Fisher Scientific Cat#B6149 CHO CD efficient feed A Thermo Fisher Scientific Cat#A1023401 ExCell 405 serum-free medium Sigma Cat#14405C 2,3-(R,R)-butandiol Alfa Aeser Cat#513-85-9 Critical commercial kits Direct-zol RNA Miniprep Plus Kit Zymo Research Cat#R2072 NucleoBond Xtra Midi Kit Macherey-Nagel Cat#12798402 NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#11992242 NucleoSpin Plasmid EasyPure Macherey-Nagel Cat#15162537 NEB Luna Universal Probe One-Step RT-qPCR kit NEB Cat#E3006S Deposited data Crystal structure of STE90-C11 in complex with SARS-CoV-2-RBD This paper PDB 7B3O Oligonucleotides Primer set for VH and VL amplification (Wenzel et al., 2020b) N/A Recombinant DNA Spike protein (RBD; S1; S1-S2; N) Thermo Fisher/Gene Art GenBank: MN908947 ACE2 Thermo Fisher/Gene Art GenBank: NM021804.3 Software and algorithms OrginPro (2019) OrginLab N/A GraphPad Prism 6.1 GraphPad https://www.graphpad.com VBASE MRC Centre for Protein Engineering https://www2.mrc-lmb.cam.ac.uk/vbase/ VBASE2 (Mollova et al., 2010) www.vbase2.org DataGraph (4.5.1) DataGraph https://www.visualdatatools.com/ DataGraph/ IncuCyte S3 GUI Sartorius N/A Phenix (Liebschner et al., 2019) www.phenix-online.org BD FACS Chorus BD N/A PyMol Schrödinger LLC www.pymol.org/2/ PISA European Bioinformatics Institute https://www.ebi.ac.uk/pdbe/ prot_int/pistart.html Octet qKe Data Analysis HT 11.0 Fortebio/Sartorius N/A Other Äkta Go/Äkta Pure Cytiva N/A Profinia System BIO-RAD N/A Precision XS microplate sample processor BioTek N/A Sartorius IncuCyte S3 Sartorius N/A BD FACSMelody Cell Sorter BD N/A Octet qKe Fortebio/Sartorius N/A PETRA III DESY Hamburg N/A Article ll OPEN ACCESS ..

    Recombinant:

    Article Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations
    Article Snippet: Reagent or Resource Source Identifier Antibodies goat-anti-hIgG(Fc)-HRP Sigma Cat#A0170; RRID:AB_257868 murine mAb 9E10 Expressed for this paper in-house, original (Chan et al., 1987) N/A goat-anti-mIgG(Fc)-HRP Sigma Cat#A0168; RRID:AB_257867 Hyper-Myc (TUN219-2C1-mFc) Abcalis Cat#ABC0503 Avelumab Expressed for this paper in-house, original (Boyerinas et al., 2015) N/A Palivizumab Expressed for this paper in-house, original (Subramanian et al., 1998) N/A STE90-C11 This paper N/A mouse anti-His epitope tag Dianova Cat#DIA-900-200 mounse anti-penta His QIAGEN Cat#34660; RRID:AB_2619735 goat anti-hIgG(Fc)-AP Jackson ImmunoResearch Cat#AB_2337599; RRID:AB_2337599 goat anti-mIgG-AP Dianova Cat#115-055-071; RRID:AB_2338535 mouse a-CD138 (FITC conjugated) BioLegend Cat#352304; RRID:AB_10900441 Bacterial and virus strains E. coli XL1 Blue MRF’ Stratagene Cat#200230 E. coli TG1 Lucigen Cat#605021 SARS-CoV-2/M€unster/FI110320/1/2020 University of M€unster, Germany N/A SARS-CoV-2 BetaCoV/Germany/ BavPat1/2020 Charité, Germany N/A Hyperphage Produced in-house N/A M13KO7 helperphage Produced in-house N/A Experimental model: cell lines Expi293F Thermo Fisher Scientific Cat#A14527 VeroE6 ATCC ATCC CRL-1586 High Five Thermo Fisher Scientific Cat#B85502 Biological samples COVID-19 patient samples local hospitals N/A COVID-19 immune libraries This paper N/A Healthy adult control samples (from preCOVID-19 time) LADR GmbH Medizinisches Versorgungszentrum Braunschweig N/A Chemicals, peptides, and recombinant proteins Spike protein (RBD; S1; S1-S2; N) This paper GenBank: MN908947 ACE2 This paper GenBank: NM021804.3 PEI 40 kDa Polysciences Cat#24765-1 Ficoll GE Healthcare Cat#45-001-749 TRIzol LS Life Technologies Cat#10296028 Superscript IV Thermo Fisher Scientific Cat#18090010 Trypsin Sigma Cat#9002-07-7 IPTG (50 mM) Sigma Cat#367-93-1 GIBCO FreeStyle F17 expression media Thermo Fisher Scientific Cat#10388033 HyClone SFM4Transfx-293 media GE Healthcare Cat#10015813 HyClone Boost 6 supplement GE Healthcare Cat#11576341 (Continued on next page) Cell Reports 36, 109433, July 27, 2021 e1 .. Reagent or Resource Source Identifier Laemmli sample buffer Laemmli, 1970 N/A 5-Brom-4-Chlor-3-Indolyl-Phosphat/Nitro Tetrazolium Blue Chloride Thermo Fisher Scientific Cat#B6149 CHO CD efficient feed A Thermo Fisher Scientific Cat#A1023401 ExCell 405 serum-free medium Sigma Cat#14405C 2,3-(R,R)-butandiol Alfa Aeser Cat#513-85-9 Critical commercial kits Direct-zol RNA Miniprep Plus Kit Zymo Research Cat#R2072 NucleoBond Xtra Midi Kit Macherey-Nagel Cat#12798402 NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#11992242 NucleoSpin Plasmid EasyPure Macherey-Nagel Cat#15162537 NEB Luna Universal Probe One-Step RT-qPCR kit NEB Cat#E3006S Deposited data Crystal structure of STE90-C11 in complex with SARS-CoV-2-RBD This paper PDB 7B3O Oligonucleotides Primer set for VH and VL amplification (Wenzel et al., 2020b) N/A Recombinant DNA Spike protein (RBD; S1; S1-S2; N) Thermo Fisher/Gene Art GenBank: MN908947 ACE2 Thermo Fisher/Gene Art GenBank: NM021804.3 Software and algorithms OrginPro (2019) OrginLab N/A GraphPad Prism 6.1 GraphPad https://www.graphpad.com VBASE MRC Centre for Protein Engineering https://www2.mrc-lmb.cam.ac.uk/vbase/ VBASE2 (Mollova et al., 2010) www.vbase2.org DataGraph (4.5.1) DataGraph https://www.visualdatatools.com/ DataGraph/ IncuCyte S3 GUI Sartorius N/A Phenix (Liebschner et al., 2019) www.phenix-online.org BD FACS Chorus BD N/A PyMol Schrödinger LLC www.pymol.org/2/ PISA European Bioinformatics Institute https://www.ebi.ac.uk/pdbe/ prot_int/pistart.html Octet qKe Data Analysis HT 11.0 Fortebio/Sartorius N/A Other Äkta Go/Äkta Pure Cytiva N/A Profinia System BIO-RAD N/A Precision XS microplate sample processor BioTek N/A Sartorius IncuCyte S3 Sartorius N/A BD FACSMelody Cell Sorter BD N/A Octet qKe Fortebio/Sartorius N/A PETRA III DESY Hamburg N/A Article ll OPEN ACCESS ..

    Software:

    Article Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations
    Article Snippet: Reagent or Resource Source Identifier Antibodies goat-anti-hIgG(Fc)-HRP Sigma Cat#A0170; RRID:AB_257868 murine mAb 9E10 Expressed for this paper in-house, original (Chan et al., 1987) N/A goat-anti-mIgG(Fc)-HRP Sigma Cat#A0168; RRID:AB_257867 Hyper-Myc (TUN219-2C1-mFc) Abcalis Cat#ABC0503 Avelumab Expressed for this paper in-house, original (Boyerinas et al., 2015) N/A Palivizumab Expressed for this paper in-house, original (Subramanian et al., 1998) N/A STE90-C11 This paper N/A mouse anti-His epitope tag Dianova Cat#DIA-900-200 mounse anti-penta His QIAGEN Cat#34660; RRID:AB_2619735 goat anti-hIgG(Fc)-AP Jackson ImmunoResearch Cat#AB_2337599; RRID:AB_2337599 goat anti-mIgG-AP Dianova Cat#115-055-071; RRID:AB_2338535 mouse a-CD138 (FITC conjugated) BioLegend Cat#352304; RRID:AB_10900441 Bacterial and virus strains E. coli XL1 Blue MRF’ Stratagene Cat#200230 E. coli TG1 Lucigen Cat#605021 SARS-CoV-2/M€unster/FI110320/1/2020 University of M€unster, Germany N/A SARS-CoV-2 BetaCoV/Germany/ BavPat1/2020 Charité, Germany N/A Hyperphage Produced in-house N/A M13KO7 helperphage Produced in-house N/A Experimental model: cell lines Expi293F Thermo Fisher Scientific Cat#A14527 VeroE6 ATCC ATCC CRL-1586 High Five Thermo Fisher Scientific Cat#B85502 Biological samples COVID-19 patient samples local hospitals N/A COVID-19 immune libraries This paper N/A Healthy adult control samples (from preCOVID-19 time) LADR GmbH Medizinisches Versorgungszentrum Braunschweig N/A Chemicals, peptides, and recombinant proteins Spike protein (RBD; S1; S1-S2; N) This paper GenBank: MN908947 ACE2 This paper GenBank: NM021804.3 PEI 40 kDa Polysciences Cat#24765-1 Ficoll GE Healthcare Cat#45-001-749 TRIzol LS Life Technologies Cat#10296028 Superscript IV Thermo Fisher Scientific Cat#18090010 Trypsin Sigma Cat#9002-07-7 IPTG (50 mM) Sigma Cat#367-93-1 GIBCO FreeStyle F17 expression media Thermo Fisher Scientific Cat#10388033 HyClone SFM4Transfx-293 media GE Healthcare Cat#10015813 HyClone Boost 6 supplement GE Healthcare Cat#11576341 (Continued on next page) Cell Reports 36, 109433, July 27, 2021 e1 .. Reagent or Resource Source Identifier Laemmli sample buffer Laemmli, 1970 N/A 5-Brom-4-Chlor-3-Indolyl-Phosphat/Nitro Tetrazolium Blue Chloride Thermo Fisher Scientific Cat#B6149 CHO CD efficient feed A Thermo Fisher Scientific Cat#A1023401 ExCell 405 serum-free medium Sigma Cat#14405C 2,3-(R,R)-butandiol Alfa Aeser Cat#513-85-9 Critical commercial kits Direct-zol RNA Miniprep Plus Kit Zymo Research Cat#R2072 NucleoBond Xtra Midi Kit Macherey-Nagel Cat#12798402 NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#11992242 NucleoSpin Plasmid EasyPure Macherey-Nagel Cat#15162537 NEB Luna Universal Probe One-Step RT-qPCR kit NEB Cat#E3006S Deposited data Crystal structure of STE90-C11 in complex with SARS-CoV-2-RBD This paper PDB 7B3O Oligonucleotides Primer set for VH and VL amplification (Wenzel et al., 2020b) N/A Recombinant DNA Spike protein (RBD; S1; S1-S2; N) Thermo Fisher/Gene Art GenBank: MN908947 ACE2 Thermo Fisher/Gene Art GenBank: NM021804.3 Software and algorithms OrginPro (2019) OrginLab N/A GraphPad Prism 6.1 GraphPad https://www.graphpad.com VBASE MRC Centre for Protein Engineering https://www2.mrc-lmb.cam.ac.uk/vbase/ VBASE2 (Mollova et al., 2010) www.vbase2.org DataGraph (4.5.1) DataGraph https://www.visualdatatools.com/ DataGraph/ IncuCyte S3 GUI Sartorius N/A Phenix (Liebschner et al., 2019) www.phenix-online.org BD FACS Chorus BD N/A PyMol Schrödinger LLC www.pymol.org/2/ PISA European Bioinformatics Institute https://www.ebi.ac.uk/pdbe/ prot_int/pistart.html Octet qKe Data Analysis HT 11.0 Fortebio/Sartorius N/A Other Äkta Go/Äkta Pure Cytiva N/A Profinia System BIO-RAD N/A Precision XS microplate sample processor BioTek N/A Sartorius IncuCyte S3 Sartorius N/A BD FACSMelody Cell Sorter BD N/A Octet qKe Fortebio/Sartorius N/A PETRA III DESY Hamburg N/A Article ll OPEN ACCESS ..

    Drug discovery:

    Article Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations
    Article Snippet: Reagent or Resource Source Identifier Antibodies goat-anti-hIgG(Fc)-HRP Sigma Cat#A0170; RRID:AB_257868 murine mAb 9E10 Expressed for this paper in-house, original (Chan et al., 1987) N/A goat-anti-mIgG(Fc)-HRP Sigma Cat#A0168; RRID:AB_257867 Hyper-Myc (TUN219-2C1-mFc) Abcalis Cat#ABC0503 Avelumab Expressed for this paper in-house, original (Boyerinas et al., 2015) N/A Palivizumab Expressed for this paper in-house, original (Subramanian et al., 1998) N/A STE90-C11 This paper N/A mouse anti-His epitope tag Dianova Cat#DIA-900-200 mounse anti-penta His QIAGEN Cat#34660; RRID:AB_2619735 goat anti-hIgG(Fc)-AP Jackson ImmunoResearch Cat#AB_2337599; RRID:AB_2337599 goat anti-mIgG-AP Dianova Cat#115-055-071; RRID:AB_2338535 mouse a-CD138 (FITC conjugated) BioLegend Cat#352304; RRID:AB_10900441 Bacterial and virus strains E. coli XL1 Blue MRF’ Stratagene Cat#200230 E. coli TG1 Lucigen Cat#605021 SARS-CoV-2/M€unster/FI110320/1/2020 University of M€unster, Germany N/A SARS-CoV-2 BetaCoV/Germany/ BavPat1/2020 Charité, Germany N/A Hyperphage Produced in-house N/A M13KO7 helperphage Produced in-house N/A Experimental model: cell lines Expi293F Thermo Fisher Scientific Cat#A14527 VeroE6 ATCC ATCC CRL-1586 High Five Thermo Fisher Scientific Cat#B85502 Biological samples COVID-19 patient samples local hospitals N/A COVID-19 immune libraries This paper N/A Healthy adult control samples (from preCOVID-19 time) LADR GmbH Medizinisches Versorgungszentrum Braunschweig N/A Chemicals, peptides, and recombinant proteins Spike protein (RBD; S1; S1-S2; N) This paper GenBank: MN908947 ACE2 This paper GenBank: NM021804.3 PEI 40 kDa Polysciences Cat#24765-1 Ficoll GE Healthcare Cat#45-001-749 TRIzol LS Life Technologies Cat#10296028 Superscript IV Thermo Fisher Scientific Cat#18090010 Trypsin Sigma Cat#9002-07-7 IPTG (50 mM) Sigma Cat#367-93-1 GIBCO FreeStyle F17 expression media Thermo Fisher Scientific Cat#10388033 HyClone SFM4Transfx-293 media GE Healthcare Cat#10015813 HyClone Boost 6 supplement GE Healthcare Cat#11576341 (Continued on next page) Cell Reports 36, 109433, July 27, 2021 e1 .. Reagent or Resource Source Identifier Laemmli sample buffer Laemmli, 1970 N/A 5-Brom-4-Chlor-3-Indolyl-Phosphat/Nitro Tetrazolium Blue Chloride Thermo Fisher Scientific Cat#B6149 CHO CD efficient feed A Thermo Fisher Scientific Cat#A1023401 ExCell 405 serum-free medium Sigma Cat#14405C 2,3-(R,R)-butandiol Alfa Aeser Cat#513-85-9 Critical commercial kits Direct-zol RNA Miniprep Plus Kit Zymo Research Cat#R2072 NucleoBond Xtra Midi Kit Macherey-Nagel Cat#12798402 NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#11992242 NucleoSpin Plasmid EasyPure Macherey-Nagel Cat#15162537 NEB Luna Universal Probe One-Step RT-qPCR kit NEB Cat#E3006S Deposited data Crystal structure of STE90-C11 in complex with SARS-CoV-2-RBD This paper PDB 7B3O Oligonucleotides Primer set for VH and VL amplification (Wenzel et al., 2020b) N/A Recombinant DNA Spike protein (RBD; S1; S1-S2; N) Thermo Fisher/Gene Art GenBank: MN908947 ACE2 Thermo Fisher/Gene Art GenBank: NM021804.3 Software and algorithms OrginPro (2019) OrginLab N/A GraphPad Prism 6.1 GraphPad https://www.graphpad.com VBASE MRC Centre for Protein Engineering https://www2.mrc-lmb.cam.ac.uk/vbase/ VBASE2 (Mollova et al., 2010) www.vbase2.org DataGraph (4.5.1) DataGraph https://www.visualdatatools.com/ DataGraph/ IncuCyte S3 GUI Sartorius N/A Phenix (Liebschner et al., 2019) www.phenix-online.org BD FACS Chorus BD N/A PyMol Schrödinger LLC www.pymol.org/2/ PISA European Bioinformatics Institute https://www.ebi.ac.uk/pdbe/ prot_int/pistart.html Octet qKe Data Analysis HT 11.0 Fortebio/Sartorius N/A Other Äkta Go/Äkta Pure Cytiva N/A Profinia System BIO-RAD N/A Precision XS microplate sample processor BioTek N/A Sartorius IncuCyte S3 Sartorius N/A BD FACSMelody Cell Sorter BD N/A Octet qKe Fortebio/Sartorius N/A PETRA III DESY Hamburg N/A Article ll OPEN ACCESS ..

    FACS:

    Article Title: A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations
    Article Snippet: Reagent or Resource Source Identifier Antibodies goat-anti-hIgG(Fc)-HRP Sigma Cat#A0170; RRID:AB_257868 murine mAb 9E10 Expressed for this paper in-house, original (Chan et al., 1987) N/A goat-anti-mIgG(Fc)-HRP Sigma Cat#A0168; RRID:AB_257867 Hyper-Myc (TUN219-2C1-mFc) Abcalis Cat#ABC0503 Avelumab Expressed for this paper in-house, original (Boyerinas et al., 2015) N/A Palivizumab Expressed for this paper in-house, original (Subramanian et al., 1998) N/A STE90-C11 This paper N/A mouse anti-His epitope tag Dianova Cat#DIA-900-200 mounse anti-penta His QIAGEN Cat#34660; RRID:AB_2619735 goat anti-hIgG(Fc)-AP Jackson ImmunoResearch Cat#AB_2337599; RRID:AB_2337599 goat anti-mIgG-AP Dianova Cat#115-055-071; RRID:AB_2338535 mouse a-CD138 (FITC conjugated) BioLegend Cat#352304; RRID:AB_10900441 Bacterial and virus strains E. coli XL1 Blue MRF’ Stratagene Cat#200230 E. coli TG1 Lucigen Cat#605021 SARS-CoV-2/M€unster/FI110320/1/2020 University of M€unster, Germany N/A SARS-CoV-2 BetaCoV/Germany/ BavPat1/2020 Charité, Germany N/A Hyperphage Produced in-house N/A M13KO7 helperphage Produced in-house N/A Experimental model: cell lines Expi293F Thermo Fisher Scientific Cat#A14527 VeroE6 ATCC ATCC CRL-1586 High Five Thermo Fisher Scientific Cat#B85502 Biological samples COVID-19 patient samples local hospitals N/A COVID-19 immune libraries This paper N/A Healthy adult control samples (from preCOVID-19 time) LADR GmbH Medizinisches Versorgungszentrum Braunschweig N/A Chemicals, peptides, and recombinant proteins Spike protein (RBD; S1; S1-S2; N) This paper GenBank: MN908947 ACE2 This paper GenBank: NM021804.3 PEI 40 kDa Polysciences Cat#24765-1 Ficoll GE Healthcare Cat#45-001-749 TRIzol LS Life Technologies Cat#10296028 Superscript IV Thermo Fisher Scientific Cat#18090010 Trypsin Sigma Cat#9002-07-7 IPTG (50 mM) Sigma Cat#367-93-1 GIBCO FreeStyle F17 expression media Thermo Fisher Scientific Cat#10388033 HyClone SFM4Transfx-293 media GE Healthcare Cat#10015813 HyClone Boost 6 supplement GE Healthcare Cat#11576341 (Continued on next page) Cell Reports 36, 109433, July 27, 2021 e1 .. Reagent or Resource Source Identifier Laemmli sample buffer Laemmli, 1970 N/A 5-Brom-4-Chlor-3-Indolyl-Phosphat/Nitro Tetrazolium Blue Chloride Thermo Fisher Scientific Cat#B6149 CHO CD efficient feed A Thermo Fisher Scientific Cat#A1023401 ExCell 405 serum-free medium Sigma Cat#14405C 2,3-(R,R)-butandiol Alfa Aeser Cat#513-85-9 Critical commercial kits Direct-zol RNA Miniprep Plus Kit Zymo Research Cat#R2072 NucleoBond Xtra Midi Kit Macherey-Nagel Cat#12798402 NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#11992242 NucleoSpin Plasmid EasyPure Macherey-Nagel Cat#15162537 NEB Luna Universal Probe One-Step RT-qPCR kit NEB Cat#E3006S Deposited data Crystal structure of STE90-C11 in complex with SARS-CoV-2-RBD This paper PDB 7B3O Oligonucleotides Primer set for VH and VL amplification (Wenzel et al., 2020b) N/A Recombinant DNA Spike protein (RBD; S1; S1-S2; N) Thermo Fisher/Gene Art GenBank: MN908947 ACE2 Thermo Fisher/Gene Art GenBank: NM021804.3 Software and algorithms OrginPro (2019) OrginLab N/A GraphPad Prism 6.1 GraphPad https://www.graphpad.com VBASE MRC Centre for Protein Engineering https://www2.mrc-lmb.cam.ac.uk/vbase/ VBASE2 (Mollova et al., 2010) www.vbase2.org DataGraph (4.5.1) DataGraph https://www.visualdatatools.com/ DataGraph/ IncuCyte S3 GUI Sartorius N/A Phenix (Liebschner et al., 2019) www.phenix-online.org BD FACS Chorus BD N/A PyMol Schrödinger LLC www.pymol.org/2/ PISA European Bioinformatics Institute https://www.ebi.ac.uk/pdbe/ prot_int/pistart.html Octet qKe Data Analysis HT 11.0 Fortebio/Sartorius N/A Other Äkta Go/Äkta Pure Cytiva N/A Profinia System BIO-RAD N/A Precision XS microplate sample processor BioTek N/A Sartorius IncuCyte S3 Sartorius N/A BD FACSMelody Cell Sorter BD N/A Octet qKe Fortebio/Sartorius N/A PETRA III DESY Hamburg N/A Article ll OPEN ACCESS ..



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